Integrated systems biology investigation (in-silico) of the hub regulatory RNA interactions (competitive endogenous RNA) in the lung cancer microarray samples

Background: Lung cancer is an insufficient treatment option, with only ۲۰% of patients responding tosystemic chemotherapy. miRNAs, discovered non-coding RNAs, regulate gene expression at the posttranscriptionallevel by base pairing with ۳’UTR of their targeted mRNAs annotated as to their targetome.Analyzing the expression mRNAs microarray from lung cancer tissues and non-tumor tissues withGSE۱۳۶۰۴۳.Result: According to the microarray data from comparing lung cancer tissue and normal tissue, we haveexamined five genes, including efcc۱, LILRB۲, JAML, SLC۱۶A۷, and DMRT۲, had the most significantexpression changes. We have found microarray data based on the highest score identified lncRNAs similarto the chromosomal number. We have examined interactions between lncRNAs and the genes. The lowexpression of our genes can lead to miR-۱۳۰۴-۵p, miR-۳۱۷۳-۳p, miR-۱۴۸a-۳p, miR-۲۲۱-۳p, miR-۷-۵, andmiR-۳۶۳-۳p not attaching to the gene, binding to the THRB-IT۱, LINC۰۱۰۰۲, LINC۰۰۶۶۵, and C۹orf۱۴۷reducing the expression of the lncRNAs. We chose three genes from five genes that indicate the existence ofCeRNA because the miRNA of genes including efcc۱, LILRB۲, and DMRT۲ had similar lncRNAs, whichindicates the existence of a complex network that can be used in gene therapy or drug. miR-۷-۵p targets agene called CCND۱ and a protein target called PA۲۸gamma. PA۲۸gamma emerges as a novel functionaltarget of tumor suppressor miRNA-۷ in non-small-cell lung cancer.Methods: All miRNAs were regained from DIANA-TarBase v۷.۰ and miRwalk databases, followed byexamining their expression in lung tumors using the GEO and DAVID database. They were findinginteraction between lncRNAs and genes by LncRRIsearch, comparing the expression of genes in lung cancerby Gepia۲ databases. The expression of lncRNAs in different tissues has been examined by the lnCARdatabases. The study of metabolic pathways and expression of the gene was performed using KEGG andReactome Pathway Database.

کلیدواژه ها:

Lung cancer ، miR-۷-۵P targetome ، Complex network ، Microarray