Development of a new methodology for Lincomycin.HCI (LINO.HCI) determination using phosphomolybdic acid (PMA) as a reagent and ISNAG-Fluorimeter Instrument via CFIA

The reaction is based on the Lincomycin that has been responded. In an acidic solution, combine HCl and phosphomolybdic acid to produce a white color precipitate that may be measured using an ISNAG-Fluorimeter (homemade instrument). Some chemical and physical variables have been evaluated. The correlation coefficient was r=۰.۹۹۸۳. The calibration curve was linear (the range was ۰.۰۰۵۱ mmol/L). The minimum detection limit concentration in the linear dynamic range of the calibration curve was ۱۹.۹۳۵ ng. The RSD percent of lincomycin HCl at ۰.۳ and ۰.۸ mmol/L was less than ۰.۲۵% (n=۸). The approach was used to determine lincomycin HCl in three pharmaceutical tablets. Comparisons were made using a standard expansion approach and a matched t-test between the recently created method and the traditional strategy (UV-Vis spectrophotometry and turbidimetry with a turbidimeter). At the ۹۵% confidence level, there was no significant difference between the two techniques.

کلیدواژه ها:

Lincomycin HCl Flow injection Scatter light ISNAG ، Fluorimeter instrument