Research Article: Extraction and characterization of collagen from the skin and bone of shabout (Arabibarbus grypus Heckel, ۱۸۴۳)

Acid soluble collagen (ASC) from the skin (ASC-S) and bone (ASC-B) of shabout (Arabibarbus grypus Heckel, ۱۸۴۳) were isolated and characterized. Both ASC-S and ASC-B from shabout contained glycine as the major amino acid and high amount of proline (Pro), hydroxyproline (Hyp), alanine and glutamic acid. On the basis of dry weight, yields of ASC-S and ASC-B were ۶.۷۹ and ۲.۵۷%, respectively. Furthermore, fourier transform infrared spectroscopy (FTIR) proved that both collagens were integrated and native. The denaturation temperature of ASC-S and ASC-B were ۳۱.۵۹ and ۳۲.۲۵°C, respectively. Additionally, the results of X-ray diffraction (XRD) proved that the two products retained their helical structures. These collagens had prominent absorptions at ۲۳۰ nm by UV-Vis spectra. Additionally, the scanning electron microscope (SEM) studies have shown that ACS-S and ASC-B are porous and exhibited fibrous nature. According to the UV–Vis and FTIR results, extracted collagens were characterized as Type I collagen based on their amino acid profile. In the current study, the total amount of amino acid (Pro+Hyp) was ۱۹.۲۸% and ۱۹.۵۵% for ASC-S and ASC-B, respectively. The results of the current study suggested that the collagen isolated from shabout can potentially be an alternative source for use in the food, pharmaceutical and biomedical industries.

کلیدواژه ها:

Shabout (Arabibarbus grypus) ، Characterization ، FTIR ، SEM skin ، bone ، Type I collagen