Study on the Antibacterial Effect from Moonmilk Pindul Cave, Indonesia

Background and Objective: Antibiotic resistance is the ability of microbes to survive antibiotic exposure with standard doses. Regular updating of antibiotics is one solution to overcome antibiotic resistance. One source with potential new antibiotics is moonmilk from caves because it contains various bacteria proven to have antibacterial activity. The objective of the present study is to explore the potential of moonmilk microbes from Pindul cave, Indonesia, as a source of new antibiotic compounds. Materials and Methods: The antibiotic potency test was divided into six groups with various concentrations of isolated moonmilk bacteria (IMM) supernatant ۲۵%, ۵۰%, ۷۵%, ۱۰۰%, negative control (-), and positive control (+). All groups were further tested for antibiotic susceptibility using the disk diffusion (Kirby and Bauer) method to pathogenic resistant bacteria Escherichia coli ATCC ۲۵۹۲۲ and Staphylococcus aureus ATCC ۲۵۹۲۳ on Mueller Hinton agar medium. PCR and Sanger sequencing method using ۱۶S rRNA primers was performed to identify species of isolated moonmilk bacteria. Results: The ۱۶S rRNA sequence analysis showed that the IMM shared a ۹۹,۶۴% (۱۳۹۹ nucleotide match) similarity with Bacillus licheniformis strain IND۷۰۶. The results showed that moonmilk bacteria had antibacterial activity against E. coli ATCC ۲۵۹۲۲ and S. aureus ATCC ۲۵۹۲۳. The highest inhibition zone was observed for the bacterial extract concentration of ۷۵% (۴۲ mm) in E. coli ATCC ۲۵۹۲۲ and ۱۰۰% (۲۳ mm) in S. aureus ATCC ۲۵۹۲۳ medium. Conclusion: IMM supernatant extract from Pindul cave, Indonesia, has the potential to control antibiotic-resistant bacterial pathogens.

کلیدواژه ها:

Antibiotics ، Bacillus licheniformis ، Moonmilk ، Pindul Cave ، آنتی بیوتیک ها ، باسیلوس لیکنیفورمیس ، Moonmilk ، Pindul Cave