Protective effect of aqueous and ethanolic extracts of Lippia citriodora Kunth. on acrylamide-induced neurotoxicity

Objective: Acrylamide (ACR) neurotoxicity is induced by different mechanisms such as oxidative stress and apoptosis. Scientific researchs have indicated the antioxidative properties of Lippia citriodora. The protective effect of L. citriodora aqueous and ethanolic extracts on ACR-induced neurotoxicity was investigated.Materials and methods: Male Wistar rats were randomly divided into ۱۳ groups: (۱) control, (۲) ACR (۵۰ mg/kg, i.p.), (۳-۶) ACR+aqueous extract (۱۲.۵, ۲۵, ۵۰, and ۱۰۰ mg/kg, i.p.), (۷-۱۰) ACR+ethanolic extract (۱۲.۵, ۲۵, ۵۰, and ۱۰۰ mg/kg, i.p.), (۱۱) aqueous extract (۱۰۰ mg/kg), (۱۲) ethanolic extract (۱۰۰ mg/kg), and (۱۳) ACR+Vitamin E (۲۰۰ mg/kg, every other day, i.p.). After ۱۱ days, gait score, MDA, and GSH levels in brain cortical tissue were measured. In the in vitro test, the viability of PC۱۲ cells (using MTT test), the amount of reactive oxygen species (ROS; using DCFH-DA method), and the protein levels of Bax, Bcl۲ and caspase ۳ (by western blotting) were measured.Results: In the in vitro study, the IC۵۰ for the treatment of PC ۱۲ cells with ACR after ۲۴ hr was ۶ mM.  ACR decreased cell viability, but increased ROS level, Bax/Bcl-۲ ratio, and caspase-۳ protein level. Pre-treatment by L. citriodora extracts (۱۵-۱۲۰ µg/ml) ameliorated the toxic effects of ACR on PC۱۲ cells. In the in vivo experiment, ACR-induced movement disorders increased MDA but decreased GSH content. The extracts of L. citriodora improved ACR toxic effects.Conclusion: Aqueous and ethanolic extracts of L. citriodora were found to reduce ACR-induced neurotoxicity via inhibiting oxidative stress and apoptosis.

کلیدواژه ها:

Acrylamide ، Lippia citriodora ، Lemon verbena ، Oxidative stress ، Apoptosis ، Aloysia citrodora