Development and validation of RP-HPLC method for simultaneous quantification of the anticancer agents, nilotinib and sorafenib: Application in In-vitro analysis

سال انتشار: 1401
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 121

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شناسه ملی سند علمی:

JR_PCBR-5-1_004

تاریخ نمایه سازی: 15 فروردین 1401

چکیده مقاله:

In this research, a reversed phase high performance liquid chromatographic method was developed for the simultaneous determination of two tyrosine kinase inhibitors, nilotinib and sorafenib. Separation was performed on an Agilent C۱۸ column (۴.۶×۲۵۰ mm, ۵µm) with mobile phase composition of potassium dihydrogen phosphate buffer (۲۵ mM, pH ۴.۲) and acetonitrile (۳۵:۶۵ v/v) at ۱.۲ mL/min with UV detection at ۲۶۵ nm. Specificity, linearity, precision, accuracy, and robustness of the proposed method were all assessed. Nilotinib and sorafenib had estimated retention times of ۵.۱ and ۵.۹ minutes, respectively. Linear concentration ranges for nilotinib and sorafenib, were determined as ۰.۰۵-۱ µg/mL and ۱۰-۴۵ µg/mL with comparable coefficient correlations (۰.۹۹۹). For nilotinib and sorafenib, the limits of detection (LOD) were determined as ۰.۰۳۰ and ۰.۰۲۰ µg/mL, while the limits of quantification (LOQ) were ۰.۱۰۱ and ۰.۰۶۹ µg/mL respectively.

نویسندگان

Azra Takhvar

Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Somaye Akbari

Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Effat Souri

Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Reza Ahmadkhaniha

Department of Human Ecology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

Ali Morsali

Department of Chemistry, Faculty of Sciences, Tarbiat Modares University, Tehran, Iran

Mohammad Reza Khoshayand

Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medial Sciences.

Mohsen Amini

Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

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  • REFERENCES [۱] M. A. Ali, Chronic myeloid leukemia in the era ...
  • J. M. Diamond, J.V. Melo, Mechanisms of resistance to BCR-ABL ...
  • T. Lahaye, B. Riehm, U. Berger, P. Paschka, M.C. Müller, ...
  • P. W. Manley, P. Drueckes, G. Fendrich, P. Furet, J. ...
  • E. Weisberg, P. Manley, J. Mestan, S. Cowan-Jacob, A. Ray, ...
  • J. Y. Blay, M. von Mehren, Nilotinib: A novel, selective ...
  • A. R. He, A. S. Goldenberg, Treating hepatocellular carcinoma progression ...
  • C. H. Takimoto, A. Awada, Safety and anti-tumor activity of ...
  • S. M. Wilhelm, C. Carter, L. Tang, D. Wilkie, A. ...
  • J. W. Panka, D. J. Wang, W. Atkins, M. B. ...
  • R. Ivaturi, T. M. Sastry, S. Satyaveni, Development and validation ...
  • A. Barla, K. Buralla, Development and validation of method for ...
  • Q. Z. Rafeeque, P. S. Dabhade, Development and method validation ...
  • A. Barla, K. K. Buralla, Development and validation of RP-HPLC ...
  • A. Rade, K. Patil, T. Thorat, P. Patil and D. ...
  • W. Talaat, M. M.Y. Kaddah, Validated analytical method for the ...
  • H. A. Alhazmi, D. Allah Moraya, E. Alahdal, M. Kariri, ...
  • I. Baranowska, B. Kowalski, Using HPLC method with DAD detection ...
  • ICH Guidelines Q۲ (R۱), Validation of analytical procedures: Text and ...
  • نمایش کامل مراجع