Anti Neuro-Inflammatory Effects of Ferulic Acid by Induction of M۲ Shift in Microglia

Objective: Ferulic acid (FA) is a natural phenolic antioxidant, which can exert also several other beneficial effects to combat neuro-inflammation and neuro-degeneration in Alzheimer's and Parkinson diseases. Microglial cells are innate immune cells that reside within the central nervous system (CNS). Activated M۱ microglia mediates some aspects of neuro-degeneration in Alzheimer's disease, while M۲ microglia which is known as good microglia reverses these toxic effects of M۱ microglia (bad microglia). In this study we examined the effects of FA, with strong anti-oxidative and anti-inflammatory activities on microglia cells.Materials and Methods: In this experimental study, microglia were isolated from ۲-day-old male mouse pup’s brain and these cells were treated with various concentration of FA (۵۰, ۱۰۰, ۲۵۰, and ۵۰۰ μg/ml) for ۲۴ hours. We assisted the following cell treatments anti-oxidant and Anti-inflammatory genes ex-pression.Results: The results revealed that FA (۵۰μg/ml) significantly increased expression of anti-inflammatory and antioxidant genes expression. It also had effects on cell morphology and cell viability.Conclusion: Based on our previous reports FA exerted neuro-protective and neuro-differentiative properties that make it a suitable choice for treatment of Parkinson and Alzheimer’s dis-eases. Our results indicate that it also has positive effects on microglial cells to activate them to express anti-inflammatory and antioxidant markers.

کلیدواژه ها:

Microglia Cell ، Ferulic Acid ، Anti-Inflammatory ، An-tioxidant