Isolation and characterization of ۱۳ new microsatellite markers in the triangle mussel (Hyriopsis cumingii)

Microsatellite enriched library of Hyriopsis cumingii was constructed according to the strong affinity between biotin and streptavidin. One hundred clones with foreign inserts were sequenced and ۶۵ clones were screened for usable microsatellites, of which ۳۶ were deemed unique, of sufficient length (more than ۸ repeats) and possessed adequate flanking regions for primer designment. Among ۳۶ primer pairs designed, ۲۵ yielded scorable amplification products. Upon testing ۳۰ individuals were sampled using the ۲۵ pair primers from Dongting Lake of Hunan Province, China. Then thirteen polymorphic microsatellite markers were isolated and characterized. These loci exhibited high levels of genetic polymorphism, so the observed number of alleles per locus ranged from ۴ to ۹. The ranges of observed and expected heterozygosity were ۰.۲۵۴۳ to ۰.۸۹۱۳ and ۰.۳۶۲۹ to ۰.۸۲۱۷, respectively, and the average polymorphic information content was ۰.۵۱۹۸. Two microsatellite loci were significantly deviated from Hardy-Weinberg equilibrium due to the presence of null alleles, and no linkage disequilibrium found. These microsatellite loci will be useful for assessing the genetic diversity and population structure of H. cumingii.

کلیدواژه ها:

Hyriopsis cumingii ، Microsatellite ، Polymorphism ، Genetic diversity