Prokaryotic expression, purification and immunogenicity analysis of CpsD protein from Streptococcus iniae

Streptococcus iniae is a major cause of serious bacterial infections in both fish and human beings. Capsular polysaccharide (CPS) of S. iniae is vital to evade phagocytic clearance of the host and serves as an important protective antigen of S. iniae infection in aquatic animals. The CpsD gene was determined to be highly conservative in capsule polysaccharide operon. Prokaryotic expression of the CpsD gene of a clinical isolate of S. iniae from channel catfish and immunogenic examination of the recombinant protein were first described in this essay. The recombinant protein was expressed in the form of inclusion bodies (IBs). Induction conditions in Escherichia coli were optimized with ۰.۶mM Isopropyl β-D-۱-Thiogalactopyranoside at ۳۷°C for ۵h after the culture mid-log phase in Luria Bertani (LB) medium. The recombinant protein CpsD was thus expressed and purified by immobilized metal affinity chromatography (IMAC), yielding approximate ۵۸۲.۴۷ mg the protein per liter culture. Western blot analysis showed that the purified CpsD had reactogenicity. It will possibly reveal more details of capsule synthesis and capsule regulation during various stages of the S. iniae infectious process.

کلیدواژه ها:

Streptococcus iniae ، Capsular polysaccharide ، Prokaryotic expression ، Purification ، Western blot analysis.