Optimization of culture conditions for growth of the Aurantiochytrium sp. shy, isolated from the Persian Gulf

In this study, a native microalgae strain with a high capability of DHA production has been isolated and purified from mangrove shores in the Persian Gulf. Morphological and molecular identification based on ۱۸S rRNA gene showed that the isolated strain belongs to Aurantiochytrium genus. In order to optimize the growth of this strain, various environmental factors were examined, including temperature (۱۵, ۲۰, ۲۵, ۳۰ and ۳۵°C), salinity (۱۰, ۳۰ and ۵۰ g L-۱) and different concentrations of nitrogen sources of yeast extract, peptone, meat extract and monosodium glutamate, each of which was tested at the concentration of ۱۰ g L-۱. The findings show that the optimal growth conditions occur at temperatures of ۲۵-۳۰ °C, pH equal to ۶ and in the presence of meat extract as the nitrogen source. In these conditions, the cellular biomass weight, fatty acids and DHA amounts obtained were ۷.۳۴, ۲.۲۵ and ۰.۴ g L-۱, respectively. These results imply that the new isolated strain of Aurantiochytrium sp. shy it can be suitable for lipid production.  

کلیدواژه ها:

Isolation ، Aurantiochytrium sp. ، Optimization ، Biomass ، Microalgae ، Heterotrophic.