Isolation and investigation of multipotent human periodontal tissue stem cells

Aim: culture of human preiodontal mesenchymal stem cells to production osteoblast. This might be used for repair of human periodontal defects in future.Method: periodontal tissues were obtained from periodontium of patients who were candidate for periodontal surgery. They were ۲۵-۴۵ years old and had no systemic diseases, no smoking, and no drug treatment. Tissues were cultured in DMEM medium. Cells were by subsequently expanded by passages. ۳ passages were done. Then cells were evaluated by inverted microscope and flowcytometry. We stained PDLstem cells with these markers: CD۴۴, CD۹۰ CD۱۶۶,CD۱۳, CD۳۴,CD۴۵ .Finding: PDL stem cells expressed MCSCs markers as shown in flowcytometry. The cells were negative by CD۳۴ and CD۴۵ markers and were positive by CD۹۰, CD۱۶۶, CD۱۳, and CD۴۴ markers .We saw a monolayer attached cells on the floor of flask macroscopically and we saw spindle cells by inverted microscope. In the microscopic finding we saw nuclear red calcified view with Alizarine staining in day ۱۴th of culture.Conclusion: Our findings show that human PDL contains a population of multypotent postnatal stem cells can be isolated and expanded in vitro. It provides a reservoir of stem cells from an accessible tissue resource. These cells have capacity of proliferation ex vivo. Therefore tissue regeneration mediated by human PDL stem cells might have potency of practical cellular- based treatment of periodontal defects.