The study of expression of CCAT۲-Lnc noncoding gene in HT-۲۹ and HCT-۱۱۶ colorectal cancer cell line and AGS gastric cancer cell line with Gemini Curcumin

Introduction: Curcumin is the main bioactive compound of turmeric that has been indicated as an impressive agent versus cancer in blocking, slowing down or reversing the carcinogenic process. However, its negligible stability and bioavailability limits therapeutic application. We formerly showed that transfer of curcumin by using Gemini surfactants nanoparticles called Gemini curcumin (Gemini-Cur) could improve its solubility, toxic trace on gastric, colorectal, breast and ovarian cancer cells. Materials and Methods: In this study, we aimed to investigate the anticancer activity of Gemini-Cur on CCAT۲-Lnc noncoding gene expression in colorectal cancer cells and gastric cancer cells. The toxicity of Gemini-Cur on HT-۲۹ and HCT۱۱۶ of colorectal cancer cells and AGS of gastric cancer cells were studied via MTT. Also, real-time PCR and western blotting were performed to evaluate the expression of the noncoding Lnc-CCAT۲ and the underlying it c-MYC genes. Results: Our data showed that Gemini-Cur enters cells quite rapidly compared to free curcumin crystals, beyond this puts down HT-۲۹, HCT-۱۱۶ and AGS cells proliferation in a time- and dose-dependent manner (p < ۰.۰۰۱) with different values at ۲۴h and ۴۸h. The IC۵۰ values as well as metastasis assays showed that Gemini-Cur decreased the expression level of the Lnc-CCAT۲ and c-MYC genes and decreased mRNA and protein level of c-MYC gene (p < ۰.۰۰۰۱). Conclusions: Generally, our findings showed that not only Gemini-Cur represses the proliferation of cancer cells but also inducts of metastasis and could be considered as novel nano-formulated phytochemical for cancer targeting.

کلیدواژه ها:

CCAT۲ ، Gemini-curcumin ، colorectal cancer ، gastric cancer ، metastasis