Some Hematological and biochemical changes in blood serum of Beluga (Huso huso) after chronic exposure to diazinon

Hematological and biochemical changes were studied in beluga (Huso huso) weighing ۴۵۰±۵۰g in the presence of  b,۱-۳, D-glucan as a single intraperitoneal injection (۰.۳mg/kg body weight) once, and thereafter a chronic exposure to organophosphate, diazinon at concentration of ۱.۵ mg/L as a continuous bath for ۶۳ days at ۲۲±۱ºC. Results of erythrocyte profile in fish treated with glucan, but without diazinon, generally showed significant higher values of RBC (P<۰.۰۵) compare to fish treated with glucan plus diazinon bath, while there was no significant difference (P>۰.۰۵) in the values of HCT, Hb, MCH and MCHC between the two groups. Also, fish treated with glucan, but without diazinon bath, showed significant higher values of RBC than that untreated fish up to ۳ weeks post-treatment (P<۰.۰۵), while no significant differences was found in other erythrocytic indices between these two groups (P>۰.۰۵). Furthermore, fish treated with diazinon showed higher levels of alkaline phosphatase (ALP), alanine aminotransferase (ALT), lactate dehydrogenase (LDH) than other three groups for ۱۴-۲۸ days post-treatment and then they decreased to the lower level for the rest of experiment. Also, fish treated with glucan-diazinon showed insignificant higher levels of these enzymes up to ۲۱ days post-exposure compare to fish treated with glucan but without diazinon bath (P>۰.۰۵). However, such levels reduced to a lower level in this group for the rest of experiment (P>۰.۰۵). Also, fish treated with glucan-diazinon showed generally lower  and higher levels of total protein and glucose concentrations in blood plasma, respectively  compared to fish treated with glucan but without diazinon bath (P<۰.۰۵). 

کلیدواژه ها:

Huso huso ، Diazinon ، Glucan ، Hematology ، Biochemistry