In Vivo oocyte maturation and ovulation in females and spermiation in males of hybrid sturgeon, bester

Bester, a hybrid sturgeon (Huso huso L. females ´ Acipenser ruthenus L. males), neither spermiate nor ovulate in the captivity. Thirteen-year-old adult male and female bester were injected with LH-RHa (۰.۱-۰.۳ mg/kg B.W.) intramuscularly and spawning status of the treated fish was checked ۲۴-۴۸ hours later. Additionally, changes in serum levels of DHP in both the responded and the non-responded individuals were monitored ۰, ۱, ۳, ۶, ۹, ۱۲, ۲۴, ۴۸ and ۷۲ hours after treatment. Between ۷۰-۱۰۰ percent of the LR-RHa injected males individuals, and ۱۱-۴۰ percent of the females spawned ۲۴-۴۸ hours after the treatment; the rest did not respond to the injection. In the responded males, ۳-۹ hours after the treatment, serum levels of DHP increased; while in the females it occurred first after ۱۲-۲۴ hours. In contrast, during the same period, serum levels of DHP remained low in all non-responded individuals. The present study indicated that using ۰.۱-۱.۳ mg/kg B. W. of LH-RHa can induce oocyte maturation and ovulation in the females and spermiation in the males cultured bester. The results suggested additionally DHP as an appropriated steroid that could be used in the final maturation stage of the gonads.

کلیدواژه ها:

Bester ، Oocyte maturation ، Ovulation ، Spermiation ، DHP ، LH-RHa