Optimization of bovine serum al bumin fibrillation: use of res ponse surf ace metho dology an d simple C ongored testing

Amyl oid fibrils are considered as nanostructures that could be formed by the self-assem bly of peptides and proteins [۱]. In recent years, amyloid fibrils are being used as experim ental nanom aterials [۲]. From this point of vie w, increasin g aggregation amount c ould be desirable. In this study, bovine serum albumin was used as a m odel protein whose aggregation process was o ptimized.Mate rials and Methods:Response surface methodology (RSM) was used i n a design that contain ed a total of ۳۰ experimental trials. The first ۲ ۴ were orga nized in a factorial design and from ۲۵ to ۳۰ involve the re plications of the central points. Data obtained from RSM w ere subjected to the analysis of variance (ANO VA) and analyzed usi ng a second order polynomial equa tion. Subsequent testing of the s uggested e xperimental parameters was done in vitro with Cong o red spectrophotometric assay [۱].

کلیدواژه ها:

Bovine Serum A lbumin ، Fibrillation ، Op timization ، Congored