Kine tic Study of Reactive Oxygen Species by Using ۲, ۷-dichlorofluoresce in Diacetate Assa y

Reactiv e oxygen sp ecies (ROS) are chemi cally reactive molecules including oxygen. Exa mples contain o xygen ions, superoxide and hydrox yl radicals. [۱]This may result in significant damage to cell structures. Generally, da mage of re active oxyg en species on the cell are most often: damage of DNA, oxidations of p olyunsatura ted fatty acids in lipids,oxidations of amino acids in proteins. In biologic al systems, use ۲, ۷-dichlorofl uorescein diacetate (DCHF-DA) for measure ment of intracellular RO S.[۲] It has been demo nstrated tha t the nonfl uorescent DCHF-DA can cross cell membranes and deacetylizes by cellular esterase enzymes and for m the nonfluor escent,oxidant-sensitive ۲,۷-dichlorofluorescein (DCHF). I n the presence of hydrogen peroxide (H۲O۲) DC HF is oxidized to highly fluorescent DCF.Purpos e of this pa er is study of mechani sm of enzy me reaction which is us ed to determ inate ROS by chemical kin etic simulation.

کلیدواژه ها:

Reactive oxygen species ، Free ra dical ، Kinetic Monte Carlo ، Simulation.