Investigation on ct-DNA thermal denaturation in interaction with Cu(Pirox)۲(DMF)۲

Piroxicam (PX) (۴-hydroxy-۲-methyl-N-۲-pridyl-۲H-۱,۲-benzothiazine-۳-carboxadiamide-۱,۱-dioxide) belongs to a class of non-steroidal pharmaceuticals with anti-inflammatory properties which are used to treat rheumatoid, arthritis and post operative inflammations[۱]. Drugs belonging to the non-steroidal anti-inflammatory drug group (NSAID) are not only used as anti-inflammatory and analgesic agents, but also exhibit chemopreventive and chemosuppressive effects on various cancer cell lines. Cu(II) complexes of these NSAIDs show better anti-cancer effects than the bare drugs. The Cu(II)–NSAID complexes have also been implicated to have better anti cancer effect [۲]. When the temperature is increased the double stranded DNA dissociates to single strands, generating hyperchromic effect. The melting temperature (Tm), a aluable parameter to study the metal complex-DNA interactions, is defined as the temperature where half of the total base pairs get unbound[۳]. The melting temperature of DNA (Tm) in the presence of a binding molecule or metal can also be used to distinguish between intercalative and external binding modes. Usually, classical intercalation gives rise to higher ¨Tm values than either groove binding or outside stacking [۴].

کلیدواژه ها:

Cu(II) piroxicam complex ، ct-DNA ، Denaturation ، Melting temperature (Tm).