UV/Vis Spectral & Viscosity Investigation of Interaction Bis ۵-Phenyl Azo Salicyl aldehyde Ethylene di imin Cobalt (II) With Calf Thymus Deoxy Ribonucleic Acid

Schiff-base ligands have been reported to show a variety of biological action by virtue of the azomethine linkage, which is responsible for various anti-bacterial, anti-fungal, anti-cancer, anti-viral, herb cal, clinical and analytical activities and this activity tends to increase in metal(II) Schiff-base complex [۱] . The chemistry of Schiff-base ligands and their metal complex has expanded enormously and encompasses a vast area of organometallic compounds and various aspect of bioinorganic chemistry [۲]. The chemistry of metal complexes containing Schiff base ligands derived from condensation of aldehydes and amines is of enduring significance, since they have common features with metalloprophyrines with respect to their electronic structure and catalytic activities that mimic enzymatic oxidation [۳].Arturo Silvestre et all , reported on the interaction of calf thymus DNA with Fe(III) Salen, that results obtained have been interpreted in terms of an electrostatic binding of [Fe(salen)]+ cation and the phosphate group of DNA [۳]. Design and synthesis of small synthetic systems that recognize specific sites of DNA is an important area of much current research these could be at least in part due to the formation of none covalently associated complexes by several such molecules with nucleic acids.In this study the interaction of CoBBASE with CT-DNA was analyzed by using spectroscopic method in various conditions as concentration and temperature, and then binding constants, binding mode, and the binding thermodynamic parameters was determined. The results obtained by UV spectroscopy with changing in temperature, DNA thermal denaturation and viscositymetery.

کلیدواژه ها:

Salen ، DNA ، UV/Vis Spectroscopy ، Viscosity ، Thermal Denaturation