Detection to trace aluminum ion of pharmaceutical wastewater using synthesis of Schiff-based chemosensor

Background: The aim of this research was to develop a fluorogenic sensor for Al۳ + ions, which have been identified as a possible food and drinking water pollutant by the WHO and considered to be harmful to human health. Methods: The sensing mechanism was based on excited-state intramolecular proton transfer, with the intramolecular rotation restriction occurring after binding with the analyte. The probe attaches Al۳ + selectively and emits strong emission in ۴:۱ H۲O/MeOH (v/v) solution while irradiated at ۴۰۰ nm in the presence of a wide number of cations, acting as a “turn-on” fluorescence chemosensor. The range of detection for Al۳ + is ۳.۳ nM (۳ method), which is more than ۲۰۰ times more responsive than the WHO suggested limit of ۷.۴ mM (۳σ method). Mass spectra, job plot, and Benesi-Hildebrand plot were used to determine the formation of the ۱:۱ metal-to-ligand complex. Results: Aluminum (Al) ion content in effluent obtained from the pharmaceutical sector is ۰.۳۸۱ mM, which is a trace amount. A separate in vitro experiment indicates that the probe can precisely perceive Al۳ + ions in a cell line. The sensor-based method is developed to detect ۳.۳ nM of Al۳ + ions, which is significantly less than the WHO max. Conclusion: The probe to detect Al۳ + ions in live cells. HL becomes a flexible sensor for recognizing intracellular Al۳ + in human liver cancer cell line Hep G۲ and human lung fibroblast cell lines by fluorescence cell imaging procedures, and the probe’s non-toxicity has been proven by MTT tests up to ۱۰۰M.

کلیدواژه ها:

Environment ، Water ، Pollution ، Poisoning ، Alzheimer’s ، Parkinson’s dementia ، Disease ، Pharmaceutical ، Aluminium ، Fluorescence chemosensor ، India