Molecular Screening of Nitrate Reductase Enzyme in Native Halophilic Bacteria of Iran

سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 248

فایل این مقاله در 9 صفحه با فرمت PDF قابل دریافت می باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

JR_JCMR-13-1_003

تاریخ نمایه سازی: 22 آذر 1400

چکیده مقاله:

With the increased usage of nitrate fertilizers, the removal of their stable ionic and water-soluble end products is a challenge for human health. Several physicochemical methods have been examined for nitrate removal of water, but biological treatments are mostly preferred due to a higher efficiency and lower cost. To remove nitrogen from water, we investigated the potential of nitrate-reducing halophilic and halotolerant bacteria. A total of ۵۰ strains from different saline and hypersaline environments of Iran, including the Incheboron wetland, Aran-Bidgol salt-lake, and Urmia endorheic salt-lake, were screened for nitrate reductase production. Among investigated bacteria, ۶۰% and ۱۹% of strains obtained from Urmia lake, and Incheboron wetland produced nitrate reductases, respectively. The nitrate reductase coding genes narG, and napA were analyzed in all strains with confirmed nitrate-reducing capacity. The napA gene was successfully amplified from a gram-negative halophilic strain, and the narG gene was detected in ten halophilic strains. Among nitrate-reducing isolates with the narG gene expression, the Kocuria rosea strain R۳A۳۴ showed the highest nitrate reductase production level. This strain was selected to optimize for its denitrifying activity. Results showed that ۳۲°C, pH ۷.۰, NaCl ۸% (w/v), and mannitol (as a carbon source) provide the optimal environmental conditions for the efficient production of nitrate reductase by the Kocuria rosea strain R۳A۳۴. As these are compatible with wastewaters conditions, this bacterium can be a proper candidate for bioremediation of wastewaters from nitrate pollutants.

کلیدواژه ها:

نویسندگان

Saeedeh Sibevieh

Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran

Ensieh Salehghamari

Department of Cell and Molecular Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran

Mohammad Ali Amoozegar

Extremophiles Laboratory, Department of Microbiology, Faculty of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran

Mohammad Reza Zolfaghari

Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran

Mohammad Soleimani

Department of Microbiology, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran.

Zohre Nasrollahzadeh

Department of Cell and Molecular Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran,

Sara Eftekhari Yazdi

Extremophiles Laboratory, Department of Microbiology, Faculty of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran

مراجع و منابع این مقاله:

لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :
  • Argandona M., Martínez-Checa F., Llamas I., Arco Y., Quesada E., ...
  • Asamoto C. K., Rempfert K. R., Luu V. H., Younkin ...
  • Baggs E. M. (۲۰۱۱). Soil microbial sources of nitrous oxide: ...
  • Baker S. C., Ferguson S. J., Ludwig B., Page M. ...
  • Beeler E., & Singh O. V. (۲۰۱۶). Extremophiles as sources ...
  • Biswas J., Bose P., Mandal S., & Paul A. K. ...
  • Boon N et al. (۲۰۱۸). Microbiome analysis and-omics studies of ...
  • Boujida N., Palau M., Charfi S., Manresa À., Senhaji N. ...
  • Cyplik P., Grajek W., Marecik R., & Kroliczak P. (۲۰۰۷). ...
  • Duan J., Fang H., Su B., Chen J., & Lin ...
  • Eltarahony M., Zaki S., & Abd-El-Haleem D. (۲۰۲۰). Aerobic and ...
  • Gao H., Yang Z. K., Barua S., Reed S. B., ...
  • He T., Xie D., Li Z., Ni J., & Sun ...
  • Javaheri-Kermani M., & Asoodeh A. (۲۰۱۹). A novel beta-۱, ۴ ...
  • Klatte T., Evans L., Whitehead R. N., & Cole J. ...
  • Kroneck P. M. (۲۰۱۸). Walking the seven lines: binuclear copper ...
  • Kushner D. J. (۱۹۸۸). Physiology of halophilic eubacteria. Halophilic bacteria. ۱۰۹-۱۳۸ ...
  • Li R., Zi X., Wang X., Zhang X., Gao H., ...
  • Luvizotto D. M., Araujo J. E., Silva M. D. C. ...
  • Ma Y., Zille , J. L., & Kent A. D. ...
  • Marmur J. (۱۹۶۱). A procedure for the isolation of deoxyribonucleic ...
  • Li R., Zi X., Wang X., Zhang X., Gao H., ...
  • Paniagua-Michel J., & Fathepure B. Z. (۲۰۱۸). Microbial consortia and ...
  • Pastorelli R., Piccolo R., Simoncini S., & Landi S. (۲۰۱۳). ...
  • نمایش کامل مراجع