Micropropagation of two apricot × plum inter specific hybrid rootstocks (HS۴۰۵ and HS۷۰۶)

Several new superior apricot × plum (Prunus armenica × P. domestica) inter specific hybrid rootstocks have been recently produced in Iran, but the propagation of these hybrids by conventional means has been shown to be very difficult. In vitro propagation could be a reliable method for mass clonal propagation of these new rootstocks. In this study, in vitro establishment, proliferation and rooting of two apricot × plum inter specific hybrid root stocks namely HS۴۰۵ and HS۷۰۶ were evaluated. For disinfection, different concentrations of ethanol, sodium hypochlorite and mercuric chloride were tested. The highest number of active buds was obtained from spring season nodal explants in hormone-free WPM medium. Addition of ۲۰۰ mg/l Na-Cefotaxime or ۱۰۰ mg/l nano silver had a positive effect on controlling indigenous contaminations. The greatest shoot proliferation was recorded by using ۴ mg/l BAP + ۰.۵ mg/l GA۳ supplemented in the WPM medium. Addition of ۲ mg/l GA۳ to the culture medium improved shoot elongation, significantly. The best results were obtained for rooting when micro shoots were dipped in a ۱۰۰۰ mg/l IBA solution for ۲۰ seconds and cultured on hormone-free WPM.

کلیدواژه ها:

Tissue culture ، Prunus ، Proliferation ، inter specific hybrid ، rootstocks