Use of microsatellite markers for molecular characterization of cumin (<I>Cuminum cyminum</I> L.) ecotypes

In this study, Simple Sequence Repeat markers (SSR) were used to investigate the genetic variation between ۴۹ cumin ecotypes collected from ۹ different provinces of Iran. SSR primers Elap۱۴۷۹, Elap۰۴۰ and Elap۱۴۹۳ showed the highest (۸۹%), while Elap۱۳۴۰ and Elap۰۱۷ showed the lowest (۵۶%) number of polymorphic bands. Polymorphism information content (PIC) values varied between ۰.۱۸ - ۰.۳۷. The highest and the lowest PIC value were obtained by Elap۰۱۷ and Elap۱۳۴۰ (۰.۳۷) and Elap۰۴۰, Elap۱۴۹۳ and Elap۱۴۷۹ (۰.۱۸), respectively. Cumin populations of Semnan and Northern-Khorasan showed the highest difference, whereas Kerman and Esfahan populations exhibited the lowest difference. The obtained dendrogram classified all cumin population into three clusters at similarity level of ۰.۷۱; the first group was Semnan and Southern-Khorasan populations, the second includes Fars, Kerman, Northern-Khorasan, Khorasan-Razavi, Esfahan, Golestan and the third class consisted of Yazd populations. Based on these clusters, Kerman and Northern-Khorasan showed the closet genetic background and may belong to the same ancestor. The genetic markers used in this study can provide impetus information for tagging of economic traits. It can be concluded that the variability in the Iranian cumin populations have potential important source for cumin breeding objectives.

کلیدواژه ها:

Cumin ، Genetic variability ، Iranian ecotypes ، SSR