Antibacterial Activity of Elephant Garlic and Its Effect against U۲OS Human Osteosarcoma Cells

  Objective(s): The present study was designed to investigate the antibacterial function and pharmacological effect of elephant garlic (Allium ampeloprasum var. ampeloprasum) on U۲OS human osteosarcoma cells.   Materials and Methods: Seven kinds of bacteria were reconstituted, inoculated and tested in this research to evaluate elephant garlic antibacterial activity. By the means of FACS analysis, cell proliferation assay, confocal laser scanning microscopy and Transwell migration assays, the effect of elephant garlic against U۲OS human osteosarcoma cells was unveiled. Rerults: The antimicrobial activity of elephant garlic was stronger than ampicillin when used against Escherichia coli, Bacillus subtilis, Bacillus thuringiensis, Staphylococcus actinomycetes, and gray actinomycetes. Even at a very low concentration (۱۲.۵%), elephant garlic still had an antibacterial effect on common bacteria E. coli and S. aureus. The G۰/G۱ ratio of elephant garlic treated group cells increased while S phase decreased. Elephant garlic extract inhibited the growth of human osteosarcoma cells, U۲OS, through preventing the transition from G۱ phase to S phase. It reduced osteosarcoma cell, U۲OS, invasion ability and significantly increased the proportion of apoptosis. It significantly affected the cytoskeleton generation. Conclusion: Elephant garlic exhibits antibacterial property and has an inhibitory effect on osteosarcoma cells (U۲OS) proliferation and cell activity, suggesting the mechanism of its anticancer effects on U۲OS human osteosarcoma cells.