The effects of Eucheuma cottonii on alveolar macrophages and malondialdehyde levels in bronchoalveolar lavage fluid in chronically particulate matter ۱۰ coal dust-exposed rats

Objective(s):To investigate the effect of Eucheuma cottonii on alveolar macrophages (AM) and malondialdehyde (MDA) levels in bronchoalveolar lavage fluids (BALF) in particulate matter ۱۰ (PM۱۰) coal dust-exposed rats. Materials and Methods: Ten groups, including a non exposed group and groups exposed to coal dust at doses of ۶.۲۵ (CD۶.۲۵), ۱۲.۵ (CD۱۲.۵), or ۲۵ mg/m۳ (CD۲۵) an hour daily for ۶ months with or without supplementation of ethanolic extract of E. cottonii at doses of ۱۵۰ (EC۱۵۰) or ۳۰۰ mg/kg BW (EC۳۰۰). The number of macrophages was determined using a light microscope. MDA levels were measured by TBARS assay. Results: EC۱۵۰ insignificantly (P > ۰.۰۵) reduces the AM in CD groups compared to non treatment groups. EC۱۵۰ and EC۳۰۰ significantly (P < ۰.۰۵) decreased MDA levels in CD۱۲.۵ and CD۲۵ groups relative to non treatment groups. Conclusion: E. cottonii attenuated oxidative stress in chronic exposure of PM۱۰ coal dust.

کلیدواژه ها:

Alveolar macrophage ، Coal dust ، Eucheuma cottonii ، Malondialdehyde