Isolation and Characterization of the Progenitor Cells From the Blastema Tissue Formed at Experimentally-Created Rabbit Ear Hole
محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 16، شماره: 2
سال انتشار: 1392
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 166
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شناسه ملی سند علمی:
JR_IJBMS-16-2_001
تاریخ نمایه سازی: 4 آبان 1400
چکیده مقاله:
Objective(s): Throughout evolution, mammalians have increasingly lost their ability to regenerate structures however rabbits are exceptional since they develop a blastema in their ear wound for regeneration purposes. Blastema consists of a group of undifferentiated cells capable of dividing and differentiating into the ear tissue. The objective of the present study is to isolate, culture expand, and characterize blastema progenitor cells in terms of their in vitro differentiation capacity. Materials and Methods: Five New Zealand white male rabbits were used in the present study. Using a punching apparatus, a ۴-mm hole was created in the animal ears. Following ۴ days, the blastema ring which was created in the periphery of primary hole in the ears was removed and cultivated. The cells migrated from the blastema were expanded through ۳ successive subcultures and characterized in terms of their potential differentiation, growth characteristics, and culture requirements. Results: The primary cultures tended to be morphologically heterogeneous having spindly-shaped fibroblast-like cells as well as flattened cells. Fibroblast-like cells survived and dominated the cultures. These cells tended to have the osteogenic, chondrogenic, and adipogenic differentiation potentials. They were highly colonogenic and maximum proliferation was achieved when the cells were plated at density of ۱۰۰ cells/cm۲ in a medium which contained ۱۰% fetal bovine serum (FBS). Conclusion: Taken together, blastema tissue-derived stem cells from rabbit ear are of mesenchymal stem cell-like population. Studies similar to this will assist scientist better understanding the nature of blastema tissue formed at rabbit ear to regenerate the wound.
کلیدواژه ها:
Blastema Differentiation Proliferation Rabbit
نویسندگان
Mohamadreza Baghaban Eslaminejad
۱Department of Stem Cells and Developmental Biology, Cell Science Research Centre, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
Sima Bordbar
۱Department of Stem Cells and Developmental Biology, Cell Science Research Centre, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
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