Antimicrobial susceptibility and analysis of macrolide resistance genes in Streptococcus pneumoniae isolated in Hamadan
محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 17، شماره: 8
سال انتشار: 1393
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 214
فایل این مقاله در 5 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_IJBMS-17-8_009
تاریخ نمایه سازی: 4 آبان 1400
چکیده مقاله:
Objective(s): Macrolide resistant Streptococcus pneumoniae pose an emerging problem globally. The aim of this study was to investigate the prevalence of ermB and mefA genes (macrolide resistant genes) by polymerase chain reaction (PCR) method and to detect drug resistance patterns of S. pneumoniae isolated from clinical samples to macrolides and other antibiotic agents by E-test method. Materials and Methods: Fifty five isolates of S. pneumoniae were obtained from clinical samples with microbial tests. The antibiotic susceptibility of isolates for erythromycin, azithromycin, clarithromycin, ceftazidime, ciprofloxacin and vancomycin were determined by E-test method. Genotypic antibiotic resistance pattern was determined by PCR with primer designed for ermB and mefA genes. Results: The number of S. pneumoniae isolates resistance to erythromycin, azithromycin, clarithromycin, ceftazidim, ciprofloxacin were ۲۵.۵%, ۱۸.۲%, ۱۶.۴%, ۲۱.۸% and ۱۰.۹%, respectively while no resistance to vancomycin was observed. The macrolide resistance genes of ermB and mefA were found in ۱۰.۹% and ۱۸.۲% of the isolates, respectively. Conclusion: The result of the current study suggests the necessity of evaluation the changes in MIC[FA۱] [a۲] (minimum inhibitory concentration) values as well as genetic mutations to estimate the prevalence of the resistance antimicrobial agents in S. pneumoniae.
کلیدواژه ها:
نویسندگان
Mohammad Najafi Mosleh
Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
Marzieh Gharibi
Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
Mohammad Yousef Alikhani
Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
Massoud Saidijam
Department of Molecular Medicine and Genetics, Hamadan University of Medical Sciences, Hamadan, Iran
Faezeh Vakhshiteh
Human and Animal Cell Bank, Iranian Biological Resource center (IBRC), ACECR, Tehran, Iran
مراجع و منابع این مقاله:
لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :