Putative mechanism for apoptosis-inducing properties of crude saponin isolated from sea cucumber (Holothuria leucospilota) as an antioxidant compound
محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 18، شماره: 2
سال انتشار: 1394
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 234
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شناسه ملی سند علمی:
JR_IJBMS-18-2_011
تاریخ نمایه سازی: 4 آبان 1400
چکیده مقاله:
Objective(s):Marine organisms are known as a potential source of natural products, which contain bioactive substances with therapeutic properties. Sea cucumbers are prominent among marine organisms because of their dietary and therapeutic applications. In addition, they have capacity of synthesizing saponins molecules and other metabolites with therapeutic properties such as antitumor, antimicrobial, anti-inflammatory and antioxidant activities. The aim of this study was to evaluate the antioxidant and pro-apoptotic effects of sea cucumber saponins (SCS) isolated from Holothuria leucospilota species. Materials and Methods: Evaluation of antioxidant activity of SCS was carried out by DPPH (۱, ۱-diphenyl-۲-picrylhydrazyl), ABTS (azino-bis-۳-ethylbenzothiazoline-۶-sulfonic acid), power reducing and total antioxidant assays. The anti-proliferative effect was studied by МТТ (۳-(۴, ۵-dimethylthiazol-۲-yl)-۲, ۵-diphenyltetrazolium bromide) assay. Mechanisms leading to apoptosis were also evaluated byfluorescence microscopy, flow cytometry and real time PCR. Results: The results showed that the DPPH and ABTS activities increased in a dose dependent manner. The reducing capacity enhanced with increasing concentration of the saponin extract (۰ to ۲ mg/ml). The SCS exhibited moderate total antioxidant activity. Evaluation of anti-proliferative effect revealed that SCS with IC۵۰ of about ۶ μg/ml, can display a good cytotoxic activity in a dose dependent manner. Further apoptosis induction was confirmed by fluorescence microscopy and flow cytometry. Sea cucumber saponin was also found to exert a pro-apoptotic effect by increasing the expression of Bax and decreasing the expression of Bcl۲. Conclusion: These results indicate that the SCS may act as a natural antioxidant and antitumor agent.
کلیدواژه ها:
نویسندگان
Mozhgan Soltani
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Kazem Parivar
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Javad Baharara
Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
Mohammad Amin Kerachian
Medical Genetics Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Javad Asili
Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
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