SstI Polymorphism of the Apolipoprotein CIII Gene in Iranian Hyperlipidemic Patients: A Study in Semnan Province

Objective(s) The Sst-I polymorphic site on the ۳' untranslated region of the apo CIII gene, has been previously reported to be associated with hypertriglyceridemia. The aim of the present study was to explore the association between Sst-I polymorphism with plasma lipid and lipoprotein levels in hyperlipidemic (HLP) patients from Semnan province, Iran. Materials and Methods Genomic DNA was prepared from ۷۶ patients with HLP and ۷۵ matched healthy subjects. DNA samples were amplified by polymerase chain reaction. The samples were analyzed by restriction fragment length polymorphism (RFLP) method using SstI enzyme. Results The genotype and allelic frequencies for this polymorphism were significantly different between HLP and normolipidemic groups (P< ۰.۰۰۲). Plasma triglyceride (TG) level was higher in both groups, in S۲S۲ genotype was more than in the S۱S۱and S۱S۲ genotypes, however, there was no significant difference in comparison with the control group. Subjects with S۱S۲ + S۲S۲ genotypes in compare to S۱S۱ genotype had odd ratio of ۲.۸ (۹۵% CI: ۱.۴۱-۵.۵۶, P< ۰.۰۰۳) for developing hypertriglyceridemia. Conclusion The results showed that the presence of rare S۲ allele was associated with change in TG level in the selected population.

کلیدواژه ها:

Apolipoprotein C-III ، Genetic ، Hyperlipidemia ، Iran ، PCR ، Polymorphism