Effect of Aqueous-Ethanolic Extract from Rosa damascena on Guinea Pig Isolated Heart

Objective(s) In the present study, the effects of aqueous-ethanolic extract from Rosa damascena on heart rate and contractility were examined. Materials and Methods Isolated guinea-pig hearts were perfused through aorta in a Langendorff mode. Heart rate (HR) and contractility were determined in the presence of four concentrations of the extract (۰.۱, ۰.۲, ۰.۴ and ۱.۰ mg %) and isoprenaline (۱, ۱۰, ۱۰۰ nM and ۱ μM) in comparison with baseline values in the presence and absence of propranolol (n= ۱۰ for each group). Results Both isoprenaline and the extract caused increase in heart rate and contractility (P< ۰.۰۵ to P< ۰.۰۰۱). The percent increased in HR due to the final concentration of isoprenaline in the absence of propranolol was significantly greater than that of the extract (P< ۰.۰۱). Propranolol caused significant reduction in both HR and contractility (P< ۰.۰۵ for both) but this effect was significantly reversed by isoprenaline and the extract (P< ۰.۰۵ to P< ۰.۰۰۱). The percent increased in heart contractility due to the final concentration of the extract in the absence and presence of propranolol was significantly greater than that of isoprenaline (P< ۰.۰۵ for both cases). There was significant correlation between both HR and heart contractility with concentration of isoprenaline and the extract (P< ۰.۰۵ to P< ۰.۰۰۱). Conclusion In conclusion this study showed a relatively potent inotropic and chornotropic effect for Rosa damascena on isolated guinea-pig heart.

کلیدواژه ها:

Aqueous-ethanolic extract ، β-adrenoceptor ، Guinea-pig ، Isolated heart ، Rosa damascena