The role of nitric oxide on the oxytocin induce analgesia in mice

Objective(s): Analgesic effects of oxytocin and it's the other physiological effects were well-known. The aim of present study was determination of nitric oxide role on analgesic effects of oxytocin in mice. Materials and Methods: ۲۱۶ male Albino mice were divided randomly into two experimental groups, tail flick and formalin test. Each experimental group consists of three main groups including: saline, L-arginine (۵۰ mg/kg) and L-NAME (۱۰ mg/kg) intraperitoneal (IP) injection. ۱۵ min after injection in each of the following groups, the animals in each groups divided to the three subgroups including:  saline (n=۱۲), oxytocin (۱ mg/kg) (n=۱۲) and oxytocin (۱ mg/kg) + atosiban (۱ mg/kg) (n=۱۲) was injected IP and then after ۳۰ min of use the formalin test and tail flick were to evaluate the response to pain. Results: Area under the curve (AUC) in the late phase of the formalin test, in sub-groups oxytocin + saline and L-NAME were significantly decreased compared with saline + saline group (P<۰.۰۵ to P<۰.۰۰۱), and AUC in L-arginine + saline and atosiban + saline + oxytocin were significantly increased compared with oxytocin + saline group (P<۰.۰۵). Tail flick tests as well as a significant reduction in the AUC in oxytocin + L-arginine and atosiban + saline + oxytocin groups were compared with Oxytocin + Saline group (P<۰.۰۰۱). Conclusion: Oxytocin has analgesic effects in the acute and late phase of pain in the formalin test. Moreover, exogenous increasing of nitric oxide reduced the analgesic effect of oxytocin.

کلیدواژه ها:

Analgesia ، Mice ، Nitric oxide ، Oxytocin