Protective effects of fractions from Artemisia biennis hydro-ethanolic extract against doxorubicin-induced oxidative stress and apoptosis in PC۱۲ cells

Objective(s): This study was designed to indicate whether different fractions from Artemisia biennis hydroethanolic extract could provide cytoprotection against oxidative stress and apoptosis induced by doxorubicin (DOX) in rat pheochromocytoma cell line (PC۱۲). Material and Methods:Cell viability was determined by MTT assay. Also, activation of caspase-۳ and superoxide dismutase were evaluated by spectrophotometry. Detection of reactive oxygen species (ROS) and measurement of mitochondrial membrane potential (MMP) were performed by flowcytometry. Results:  Treatment of PC۱۲ cells with DOX reduced viability dose dependently. For evaluation of the effect of fractions (A-G) on DOX-induced cytotoxicity, PC۱۲ cells were pretreated for ۲۴ hr with the A. biennis fractions and then cells were treated with DOX.  The fractions C and D increased PC۱۲ cells viability significantly compared to DOX treated cells.  Moreover, pretreatment with fractions C and D for ۲۴ hr attenuated DOX-mediated apoptosis and the anti-apoptotic action of A. biennis fractions was partially dependent on inhibition of caspase ۳ activity and also increasing the  mitochondrial membrane potential (MMP). Selected A. biennis fractions also suppressed the generation of ROS and increased superoxide dismutase (SOD) activity. Conclusion: Taken together our observation indicated that subtoxic concentration of aforementioned fractions of A. biennis hydroetanolic extract has protective effect against apoptosis induced by DOX in PC۱۲ cell. The results highlighted that fractions C and D may exert cytoprotective effects through their antioxidant actions.

کلیدواژه ها:

Apoptosis ، Artemisia biennis ، Doxorubicin ، Oxidative stress ، PC۱۲ cell line