Sertoli cell condition medium can induce germ like cells from bone marrow derived mesenchymal stem cells

Objective(s): Although many researchers have confirmed induction of germ cells from bone marrow mesenchymal stem cells (BMMSCs), there are no reports that confirm spontaneous differentiation of germ cells from BMMSCs. In this study, we have evaluated the effect of adult Sertoli cell condition medium (SCCM) as a mutative factor in the induction of germ cells from BMMSCs. Materials and Methods: BMMSCs were collected from the bone marrow of ۶-۸-week old NMRI mice and their mesenchymal entities were proven using superficial markers (expression of CD۴۴ and CD۷۳ and non-expresion of CD۴۵ and CD۱۱b) by fow cytometry. Their multi-potential entities were proved with differentiation to osteogenic and adipogenic cells for ۲۱ days. Also isolated Sertoli cells were enriched using lectin coated plates and Sertoli cell condition medium (SCCM) was collected. Sertoli cells were identified by immunocytochemistry and Vimentin marker. The cells were then differentiated into germ cells with SCCM for ۲ weeks. Finally induced cells were evaluated by RT-PCR and immunocytochemistry. Results: Differentiation of mesenchymal stem cells to osteoblast and adipocyte showed their multi-potential property. Expression of CD۴۴ and CD۷۳ and non-expression of CD۴۵ and CD۱۱b confirmed mesenchyme cells. Immunocytochemistry and RT-PCR results showed expression of germ cells specific marker (Mvh). Conclusion: This study confirmed the effect of SCCM as a motivational factor that can used for differentiation of germ cells from BMMSCs.

کلیدواژه ها:

Bone marrow mesenchymal stem cells ، Differentiation ، Germ cells ، Sertoli cell condition medium