Effect of genistein on expression of pancreatic SIRT۱, inflammatory cytokines and histological changes in ovariectomized diabetic rat
محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 20، شماره: 4
سال انتشار: 1396
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 236
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شناسه ملی سند علمی:
JR_IJBMS-20-4_011
تاریخ نمایه سازی: 28 مهر 1400
چکیده مقاله:
Objective(s): Genistein is reported to have anti-diabetic and anti-inflammatory functions, in particular, direct effects on β-cell proliferation and insulin secretion. In this study, we investigated the anti-inflammatory effect of genistein on the pancreatic β-cells in ovariectomized diabetic rat. Materials and Methods:Forty female rats were divided into four groups: sham, bilateral ovariectomy (OVX), OVX.D (OVX+diabetes) and OVX.D.G (OVX.D+genistein). After bilateral ovariectomy, rats in the diabetic groups were fed high-fat diet (HFD), ad libitum for ۴ weeks, and then a low dose of streptozotocin (STZ) (۳۰ mg/kg) injected intraperitoneally. Genistein (۱ mg/kg/day; SC) was administrated for ۸ weeks. At the end of ۸ weeks, pancreas tissue was removed and used for western blotting and Hematoxylin-Eosin staining. Results: Treatment with genistein declined inflammation and tissue injury, and this decline was correlated with the expression of SIRT۱. OVX and OVX.D significantly increased Nf-кB and IL-۱β expression and decreased SIRT۱ levels compared to sham group (P<۰.۰۵). Significant reduction of Nf-кB and IL-۱β, and increasing of SIRT۱ were observed during genistein treatment (P<۰.۰۵). Conclusion: Estrogen deficiency alone or with HFD increased pancreatic inflammation. However, subcutaneous administration of gtenistein prevented from these inflammatory changes in the pancreas of a surgery animal model of ovariectomy with or without diabetes. Our results support the potential preventing effect of genistein from pancreatic injury.
کلیدواژه ها:
نویسندگان
Hadi Yousefi
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Alireza Alihemmati
Department of Histology & Embryology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Pouran Karimi
Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Mohammad Reza Alipour
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Parisa Habibi
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Nasser Ahmadiasl
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
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