Comparison of the effects of ۱۷β- estradiol treated and untreated mesenchymal stem cells on ameliorating animal model of multiple sclerosis

Objective(s): The current investigation was undertaken to evaluate the effects of ۱۷β- estradiol (۱۷β-ED) on the potential of the mesenchymal stem cells (MSCs) for modulation of immunity responses in an animal model of multiple sclerosis (MS).Materials and Methods: After isolation of MSCs, cells were cultured in presence of ۱۰۰ nM ۱۷β-ED for ۲۴ hr. Modeling of experimental autoimmune encephalomyelitis (EAE) was achieved by using guinea pig spinal cord homogenate, in addition to complete Freund’s adjuvant in male Wistar rats. The processes of cell therapy were started following ۱۲ days post-immunization. This duration allows all animals to develop a disability score. The achieved EAE clinical symptoms were regularly monitored every day until day ۳۶, when all of examined rats were euthanized. Results: Cell therapy in the EAE rats with ۱۷β-ED-primed MSCs exhibited more desirable consequences, which in turn lead to regression of the cumulative clinical score and neuropathological changes that are more than the therapy with untreated MSCs. The serum measures of myeloperoxidase (MPO), nitric oxide (NO) as well as splenocytes-originated pro-inflammatory interleukin-۱۷ (IL-۱۷) and tumor necrosis factor alpha (TNF-α) were significantly decreased in EAE rats treated by ۱۷β-ED primed-MSCs compared to EAE rats that received untreated MScs. Conclusion: Combination of ۱۷β-ED and MSCs more effectively improved the signs and symptoms of EAE.

کلیدواژه ها:

Experimental autoimmune encephalomyelitis ، Immunotherapy ، Mesenchymal stem cell ، Multiple Sclerosis ، ۱۷β- estradiol