Iranian crack induces hepatic injury through mitogen-activated protein kinase pathway in the liver of Wistar rat
محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 21، شماره: 11
سال انتشار: 1397
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 216
فایل این مقاله در 7 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_IJBMS-21-11_013
تاریخ نمایه سازی: 27 مهر 1400
چکیده مقاله:
Objective(s): Iranian crack (IC) is a heroin-based substance manifesting various pathologic side effects. Herein, we aimed to investigate the mechanism of IC-induced liver injuries in Wistar rats. Materials and Methods: Twenty male Wistar rats were randomly divided into two groups: control, and IC (۰.۹ mg/kg/day/IP, for ۳۰ days). Mitochondrial reactive oxygen species (ROS) production was measured by DCF fluorescence staining. The expression of tumor necrosis factor-alpha (TNF-α), interleukin ۱β (IL-۱β), and phosphorylation of p۳۸ mitogen-activated protein kinase (p۳۸ MAPK) and c-Jun N-terminal kinase (c-JNK) were assessed by immunoblotting assay. The intensity of collagen fiber in the liver was also determined by Trichrome-Masson staining. Furthermore, serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) activities were measured using colorimetric methods. Results: Our results showed that ROS production, p۳۸ MAPK, c-JNK phosphorylation levels, and expression of TNF-α and IL-۱β were significantly elevated in the liver tissue of IC group as compared to the control group. Moreover, collagen fiber and ALT activity were increased in the liver tissue of IC group compared to the control group. However, there was no statistically significant difference in the levels of ALP between two groups. In addition, there was a positive correlation between the intensity of collagen fiber and the ALT activity, and the levels of TNF-α and IL-۱β and liver enzymes activities including ALP, ALT, and AST.Conclusion: Our findings revealed that IC-induced liver cells injury is partially mediated by MAPK stress kinases. Therefore, regular liver examination in substance abuse is strongly recommended.
کلیدواژه ها:
نویسندگان
Aliasghar Parvaresh Anbar
Higher Academic Education Institute of Rab-e Rashid, Tabriz, Iran
Tayyebeh Piran
Higher Academic Education Institute of Rab-e Rashid, Tabriz, Iran
Mehrdad Farhadi
Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Pouran karimi
Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
مراجع و منابع این مقاله:
لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :