Saffron offers hepatoprotection via up-regulation of hepatic farnesoid-X-activated receptors in a rat model of acetaminophen-induced hepatotoxicity

سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 318

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شناسه ملی سند علمی:

JR_AJP-11-6_008

تاریخ نمایه سازی: 27 مهر 1400

چکیده مقاله:

Objectives: The most important toxicity of acetaminophen is hepatotoxicity. Farnesoid X-activated receptors (FXR) are one of the nuclear receptor superfamily members which have a pivotal role in the bile acid regulation. The objective of the present study was to examine the role of FXR in mediating the hepatoprotective effects of saffron.Methods: Male Wister rats were randomly allocated into five groups including a control, vehicle, acetaminophen and two saffron extract groups of ۱۵۰ and ۳۰۰ mg/kg/day. The liver function and hepatic FXR expression were evaluated using biochemical assay and real time RT-PCR, respectively. Data analysis was performed using the one-way ANOVA followed by Duncan's multiple range test.Results: Levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) of the acetaminophen group were significantly higher than the control group whereas those of the extract-treated groups were significantly lower than those of the acetaminophen group. The real time RT-PCR findings showed a non-significant down-regulation of FXR mRNA expression, however, a dose-dependent FXR up-regulation was seen in the groups treated with ۱۵۰ and ۳۰۰ mg/kg of the extract for ۲.۶۷ (p=۰.۰۰۲) and ۱۰.۲۲ (p=۰.۰۰۰۱) fold, respectively.Conclusion: The main finding of the present study was that the hepatic FXR up-regulation had an important role in saffron hepatoprotective activity.

نویسندگان

Vahid Jamshidi

Department of Pharmacology and Toxicology, Faculty of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran

Seyed Ali Hashemi

Department of Pathology, School of Medicine, Alborz University of Medical Sciences, Karaj,Iran

azadeh Khalili

Department of Physiology-Pharmacology-Medical Physic, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran Evidence-based Phytotherapy and Complementary Medicine Research Center, Alborz University of Medical Sciences, Karaj,

Parviz Fallah

Department of Medical Laboratory Sciences, Faculty of Para-Medicine; Alborz University of Medical Sciences

Mohammad Mahdi Ahmadian-Attari

Department of Pharmacognosy, Faculty of Pharmacy, Alborz University of Medical Sciences, Karaj,Iran Evidence-based Phytotherapy and Complementary Medicine Research Center, Alborz University of Medical Sciences, Karaj, Iran

Leila Beikzadeh

Department of Medical Laboratory Sciences, Faculty of Para-Medicine, Alborz University of Medical Sciences, Karaj, Iran

Roham Mazloom

Department of Physiology-Pharmacology-Medical Physic, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran

Parvaneh Najafizadeh

Department of Pharmacology and Toxicology, Faculty of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran Department of Pharmacology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

Gholamreza Bayat

Department of Physiology-Pharmacology-Medical Physic, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran