Identification of Celecoxib targeted proteins using thermal proteome profiling on rat hippocampus

The search for disease-related targets and the study of drug-protein interaction and protein-protein interactions are central issues that would accelerate the clinical approval of a drug and decrease the time and resources consuming in this regard. The less drug efficiency on the human is a grave issue in drug discovery that causes the low rate of FDA (Food and Drug Administration) approval after spending billions of dollars and spending decades on it. This limitation causes to develop a new technique, Thermal Proteome Profiling (TPP). The TPP is based on the combination of the Cellular Thermal Shift Assay (CETSA) and quantitative mass spectrometry (۱). Celecoxib is one of the NSAID's non-steroidal anti-inflammatory drugs. It is one of the most prevalent medicines (۲), which despite the benefits of treating inflammatory diseases, is associated with unwanted side effects, and its targets in the nervous system are not well understood (۳-۵). We developed this project to identify targets of Celecoxib through the TPP technique. In this research, we identified seven targeted proteins in rat hippocampus, which are enrolled in different pathways and diseases such as neurodegenerative disease.

کلیدواژه ها:

drug repurposing ، drug target ، thermal proteome profiling ، celecoxib