Correlation between DNMT۱ gene֝s expression and Helicobacter pylori virulence genes

Background and Aim : DNMT۱ contributes to the cell-critical gene expression by methylation of DNA cytosine-rich sequences. Environmental factors, including Helicobacter pylori infection, change host cells' epigenetics by affecting DNMT۱ gene expression. This study investigates the correlation between H. pylori virulence genes and DNMT۱ gene expression in gastric epithelial cells of gastric adenocarcinoma and gastritis patients. Methods : In a case-control study, ۵۰ and ۵۳ gastric antral biopsy specimens from patients with gastritis and gastric adenocarcinoma were evaluated, respectively. The urea breath test results showed that ۲۳ and ۲۱ patients with gastritis and gastric adenocarcinoma had H. pylori infection, respectively. After RNA extraction from gastric biopsy samples and cDNA synthesis, virulence genes and EBER of H. pylori and Epstein-Barr virus were detected by PCR. Relative Real-Time RT PCR used to detect the DNMT۱ gene expression and fold changes in groups of patients were surveyed by ΔΔCt. Results : The results showed that with increasing age and the H. pylori cagA, cagY, and cagE genotypes, the DNMT۱ gene expression increases in patients' gastric epithelial cells gastric cancer (p <۰.۰۵).Conclusion : Increased expression of DNMT۱ induced by H. pylori with epigenetic effect on host cells is involved in the increased risk of gastric cancer in people with H. pylori .

کلیدواژه ها:

Helicobacter pylori ، DNMT۱ gene ، epigenetics ، gastric adenocarcinoma ،