sirt۳, ۶, and ۷ genes֝ expression in gastric antral epithelial cells of patients with Helicobacter pylori infection

Background and Aim : As the intracellular acylation enzymes, Sirtuins play a crucial role in regulating the cell's physiological activities. Previous studies have shown a different sirtuin genés expression in types of cancer. This study has surveyed transcription of sirt۳, ۶, and ۷ genes in gastric antral epithelial cells of gastritis and gastric adenocarcinoma patients with and without H. pylori infection. Methods : A case-control study was conducted, including ۵۰ and ۵۳ gastric antral biopsy samples collected from gastritis and gastric adenocarcinoma patients with and without H. pylori infection referred to hospitals of Sanandaj city during ۲۰۱۸-۲۰۱۹. Total RNAs were extracted from biopsy samples, then cDNAs were synthesized by using Takara kits. Quality essay of H. pylori virulence genés expression and relative quantitative essay of sirt۳, ۶, and ۷ genés expressions in gastric antral biopsy samples were performed using the Real-Time RT PCR method. Results : The statistical correlations were observed between H. pylori vacA s۱m۲ and sabA cDNA with gastric antral epithelial cells sirt۳ geneś expression [p=۰.۰۵, ۰.۰۲۳ respectively]. sirt۶ geneś expression decreased with age increasing in gastric adenocarcinoma patients [p=۰.۰۳]. The cDNA of H. pylori hopQII, oipA, and sabB were companied with gastric antral epithelial cells sirt۷ geneś expression of gastritis patients [p=۰.۰۳۱, ۰.۰۴۸, and ۰.۰۴۲ respectively]. Conclusion : In conclusion, the expression of H. pylori virulence genes and age increasing of patients have correlations with gastric antral cells sirt۳, ۶, and ۷ geneś expressions of patients.

کلیدواژه ها:

Helicobacter pylori ، sirt۳ ، sirt۶ ، sirt۷ ، Gastric Cancer ، Gastritis