Transcriptional Profile of Helicobacter pylori virulence genes in patients with gastritis and gastric cancer

Background and Aim : Numerous molecular epidemiology studies have been done about the frequency of Helicobacter pylori virulence genes in patients with H. pylori infection so far. This study was conducted to detect transcriptional profile by cDNA of H. pylori virulence genes in gastric biopsy samples of gastritis and gastric carcinoma patients.Methods : In a case-control study, based on the prevalence of gastritis and gastric cancer in Sanandaj city during ۲۰۱۸ and ۲۰۱۹, ۲۳ and ۱۱ gastric antral biopsy samples with H. pylori infection were collected from gastritis and gastric carcinoma patients by consecutive and available sampling method. Pathological characters, including tumor grades and tumor areas for gastric carcinoma biopsy samples prepared from gastric cancer areas, were determined by the pathologist. Total RNA of gastric antral biopsy samples was extracted, and their cDNA was synthesized by TaKaRa kit. Detection of H. pylori virulence genes’ cDNA using specific primers and PCR. This study's results were analyzed by SPSS version ۲۵ and statics chi-square tests for determination of relationship and correlation between cDNAs of H. pylori transcriptional profile and clinical outcomes of H. pylori infection, including gastritis, gastric carcinoma, tumor grades, and tumor area.Results : The positive statistical correlations were observed between transcripts of cagA, cagA- EPIYAC, cagE, and cagY genes and H. pylori infection clinical outcomes (P<۰.۰۵).Conclusion : Detection of the H. pylori virulence genes’ cDNA in gastric biopsy samples can help provide the prognosis of clinical outcomes.

کلیدواژه ها:

Helicobacter pylori ، Gene Expression Profiling ، Virulence Factors ، cDNA ، Gastric Cancer ، Gastritis