Herpes simplex type ۱ virus inactivation using silver nanoparticles

Background and Aim : Silver nanoparticles have been used against wide range of viruses and they have shown low possibility to be resisted by viruses, comparing to other common antiviral agents. Silver nanoparticles consist of two main parts which are silver salts and capping agents which make them different from each other and showing different features. herpes simplex type ۱ virus belongs to herpes viridae family which is enveloped and has double stranded DNA genome. These two features can make the virus, a model for viruses which has common properties with it. So silver nanoparticles probable antiviral activity against the virus can be used for a range of viruses with those two common properties. Methods : In this study, AgNO۳ and aqueous extract of dried Juglans regia green husk play the role of two main components to make silver nanoparticles. Silver nanoparticle cytotoxicity examined on Vero cell line and the maximum non toxic dose was ۱۰ µg/ml that shows this nanoparticle is so toxic. Silver nanoparticle prepared in that low concentration and tested on the virus but it didn’t have any antiviral effect and Cytopathic effect was seen. For using highest stable concentration of silver nanoparticle which is ۳۰۰ µg/ml , magnetic Fe nanoparticles get involved so silver nanoparticle in that toxic dose could be gathered before being exposure to cells and cause their death. Results : In this method, treated viruses exposure to cells and virus titre decreased without any nanoparticles toxicity. Both magnetic Fe nanoparticle and silver nanoparticle showed antiviral activity by their own but their result on virus titre reduction enhanced when they used together and they showed synergism effect. Both nanoparticles caused at least ۴.۵ log inactivation.Conclusion : Silver nanoparticle reduced herpes simplex type ۱ virus titration before getting exposure to cells and this give the possibility of effecting on virus attachment stage. Due to silver nanoparticles variable synthesis methods and sizes, the exact mechanism of them is not clear.

کلیدواژه ها:

Antiviral ، Disinfectant ، Herpes simplex type ۱ virus ، Silver nanoparticles