Polio virus inactivation using silver nanoparticles

Background and Aim : Antiviral drug resistance these days becomes an important issue which led us to antiviral agents that can be effective against resistant strains of viruses. Silver nanoparticles count as one of these unresistable agents and many researches has done about their antiviral activity. There are different kinds of silver nanoparticles due to their synthesis methods and their components. In this study, biosynthesized silver nanoparticle with aqueous extract of dried Juglans regia green husk antiviral activity estimated against polio virus. This virus belongs to Picornaviridae family and is a sample for viruses without envelope and/or whit RNA genome.Methods : Silever nanoparticle cytotoxicity examined through MTT assay and the nanoparticles was so toxic to be exposured Vero cell line in high stable concentrations that can show antiviral activity so gathering them after affecting on viruses becomes a solution. Viruses treated with the highest ۲۴ hours stable concentration of silver nanoparticle which is ۳۰۰ µg/ml and then added to magnetic Fe nanoparticles therefore they could be gathered using magnet. Treated virus exposure to cell without any cytotoxicity.Results : Polio virus titration reduced after being treated with both silver nanoparticle and magnetic Fe nanoparticle that showed both of nanoparticles had antiviral activity and they enhanced each others through synergism mechanism and they resulted in at least ۴ log inactivation.Conclusion : Silver nanoparticle and magnetic Fe nanoparticle could use as an antiviral agents but using high stable concentrations of silver nanoparticle is possible when it used with magnetic Fe nanoparticle because of its cytotoxicity. Using both of nanoparticles could decrease polio virus titration without existing in the medium after treating the virus.

کلیدواژه ها:

Antiviral ، Disinfectant ، Polio virus ، Silver nanoparticles