Prevalence of ESBL Genes in Clinical Isolates of Pseudomonas aeruginosa from Burn Patients in Isfahan, Iran
محل انتشار: بیست دومین کنگره میکروب شناسی ایران (مجازی)
سال انتشار: 1400
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 237
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شناسه ملی سند علمی:
MEDISM22_320
تاریخ نمایه سازی: 8 مهر 1400
چکیده مقاله:
Background and Aim : Pseudomonas aeruginosa is very common in burn wound infections. The biofilm formation and the production of beta-lactamase enzymes have made this bacterium resistant to many antibiotics. This study aimed to evaluate antibiotic resistance, biofilm formation, and the frequency of strains that carry blaTEM and blaVEB.Methods : A cross-sectional study was carried out from March ۲۰۱۷ to March ۲۰۱۸ on ۱۰۳ clinical isolates of P. aeruginosa, identified from ۴۲۰ burn wound infection samples by phenotypic tests. Antibiotic resistance and biofilm formation were evaluated by the Kirby-Bauer disk diffusion method and microtiter plate assay, respectively. Double disk synergy test (DDST) was used for phenotypical detection of Extended-spectrum beta-lactamases (ESBL) producing isolates. ESBL genes were detected by polymerase chain reaction (PCR).Results : Of ۱۰۳ strains isolated from burn wound infection, ۹۱.۳% were multiple drug resistance (MDR). The resistance to levofloxacin was the highest (۹۳.۲%). The ability of biofilm formation was observed in three groups: ۴۷.۶% of isolates had no biofilm formation, ۳۸.۸% were weak, and ۱۳.۶% showed moderate biofilm formation. The prevalence of DDST-confirmed ESBL was ۵۴ (۴۳.۹%). The isolates had ۲۸.۱۵% and ۲۱.۳۵% of blaVEB and blaTEM genes, respectively. Conclusion : This study showed that MDR strains are common in burn infections. Biofilm formation and produce beta-lactamase enzymes also showed a growing trend.
کلیدواژه ها:
نویسندگان
Elham Haghighifar
Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Razie Kamali Dolatabadi
Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Fatemeh Norouzi
Instructor, Department of Microbiology, School of Medicine, Fasa University of Medical Sciences