the study of optimal drying method of Keratinase Enzyme With the aim of maintaining its efficiency

Background and Aim : Keratinase is an extracellular endopeptidase moreover maximal activity in its particular keratin substrate has the capacity to degrade a wide range of substrates such as casein, collagen, elastin, globulin, and hemoglobin and in numerous industries including pharmaceuticals, Leather industry, livestock, insecticides, prion decomposition, cosmetics, and organic agricultural fertilizers, it also functions in a diversity of temperature and PH ranges. The aim of this study is to investigate the optimal method of drying the enzyme to maintain its activity by oven, vacuum oven, and freeze dryer.Methods : In the first instance, after cultivating bacteria for four days in an alkaline medium containing feathers and salt, complete decomposition of the feathers Took place.Afterwards, with the intention of separating the feathers and bacteria from the medium, centrifugation was performed and the enzyme in the supernatant was precipitated by ammonium sulfate salt.Eventually, after the final centrifugation, the precipitate containing the enzyme was dried in freeze dryer, oven, and vacuum oven, and the keratinase and protease activity of the samples were measured.Results : The keratins activity of dried samples in an oven, vacuum oven and freeze dryer by feather substrate, respectively; ۶.۵ (U /ml), ۶.۸ (U / ml) and ۲۵.۵ (U / ml) were measured. Also,protease activity of the same samples by casein substrate, respectively; ۱۲.۷ (U / ml), ۱۳ (U / ml) and ۹۷ (U / ml) were measured.Conclusion : Considering the numbers obtained from the activity of the enzyme after drying per all three methods, it is concluded that drying the enzyme by a freeze dryer clearly preserves the keratinase and protease activity of the enzyme compared to the other methods.

کلیدواژه ها:

Protease ، keratinase ، freeze dryer ، oven ، vacuum oven