Assessment of the anti-biofilm capacity of Lactobacillus plantarum strains isolated from kefir dough against streptococcus mutans

Background and Aim : The Streptococci are the pioneer strains in plaque formation and Streptococcus mutans is the main etiological agent of dental plaque and caries. Probiotics can release bioactive substances that can inhibit the growth and biofilm formation of pathogenic microorganisms. Among of those probiotic bacteria Lactobacillus plantarum has been proposed that boost oral health. The present study focus on determination of the L. plantarum supernatants on biofilm formation of S. mutans (ATCC۳۵۶۶۸).Methods : In this experimental study, we isolated ۵ strains of L. plantarum from local kefir dough of Isfahan region were used as probiotic which were cultured at ۳۷°C in anaerobic condition, then identified using morphological characteristics, biochemical tests and PCR technique. Biofilm formation was detected by crystal violet assay using ۹۶ well microtiter plate. Results : All ۵ Lactobacillus strains showed anti-biofilm activity on S. mutans. L. plantarum which isolated from five different sources demonstrated variable effect on biofilm from five times reduction to nine times. They demonstrated significantly reduction on biofilm.Conclusion : It seems that supernatant of the probiotics isolated from kefir dough can be used as an antibiotic alternative to decrease the chance of dental caries.

کلیدواژه ها:

Supernatant ، Streptococcus mutans ، Kefir dough ، Probiotics ، Lactobacillus plantarum ، Anti-biofilm