Isolation and Characterization of Effective Probiotics against Drug-Resistant Acinetobacter Strains

سال انتشار: 1400
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 239

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شناسه ملی سند علمی:

MEDISM22_257

تاریخ نمایه سازی: 8 مهر 1400

چکیده مقاله:

Background and Aim : Today, with the increase of nosocomial infections caused by opportunistic gram-negative pathogens such as Acinetobacter and the indiscriminate use of antibiotics, multidrug resistance has increased, following the increase in antibiotic resistance and side effects. From the use of chemical drugs, the use of alternative therapies seems necessary. Therefore, probiotics and their metabolites, including organic acids and bacteriocins, can be widely used in therapeutic applications. The emergence of antibiotic-resistant and foodspoilage microorganisms has renewed efforts to identify safe and natural alternative agents of antibiotics such as probiotics. The aim of this study was to Isolation and Characterization of Effective Probiotics against Drug-Resistant Acinetobacter Strains. Methods : First, over a period of six months, ۱۰۰ samples of Acinetobacter were collected from four main hospitals in Isfahan, and after determining the antibiotic susceptibility test in them, ۵۳ strains of Acinetobacter were resistant to carbapenem and ۶ strains of Acinetobacter were resistant to colistin. The antimicrobial effect of ۲۰ different probiotic bacteria isolated from dairy products against Acinetobacter was investigated by diffusion method in plate well and biofilm formation was investigated by three different methods.Results : The results showed that one probiotic strain isolated from local yogurt in Isfahan province was resistant to all strains of Acinetobacter and by hot staining it was determined that the bacterium Lactobacillus and the colony form is a non-diphtheria bacillus. The lethal and inhibitory effects of this probiotic on the killing of resistant strains were confirmed. The results showed that this probiotic killed pathogenic bacteria only after ۱ hour and its inhibitory mechanism was due to organic acids such as lactic acid and acetic acid.It was resistant to bile acid and low pH tolerance. Various pathogenicity tests, including catalase, hemolysis, CAMP, Dnase, and antibiotic susceptibility to the probiotic strain indicate lack of pathogenicity and lack of antibiotic resistance.Conclusion : The results were demonstrated by high performance liquid chromatography (HPLC) analysis Its inhibitory effect was due to the production of two main organic acids including lactic acid and acetic acid. Due to the widespread activity of this strain, it can be potentially used in the biological control of drug-resistant Acinetobacter strains.

نویسندگان

Zahra Abbasi

Department of Biotechnology, Faculty of Biological Sciences and Technology, Shahid Ashrafi Esfahani University

Dariush Shokri

Department of Biotechnology, Faculty of Biological Sciences and Technology, Shahid Ashrafi Esfahani University

Seyyed Mahdi Ghasemi

Department of Biotechnology, Faculty of Biological Sciences and Technology, Shahid Ashrafi Esfahani University