Biochemical and Serological Study of Vibrio Bacteria in Surface Water of Khuzestan Province Before and After the Spring Floodwaters of ۲۰۱۹

Background and Aim : Vibrio genus are gram-negative, motile, oxidase-positive, halophilic bacteria found in seawater, oceans, as well as in surfaces waters and rivers. This genus is a part of Microbial population of environmental waters and has been isolated from sediments, and seafoods . More than a hundred species of this genus have been identified, at least ۱۲ species in this genus are pathogenic to humans .Vibrio cholerae is type species, that causes cholera disease in humans. The aim of this study was biochemical and serological diagnosis of Vibrio species in surface waters of Khuzestan province before and after the ۲۰۱۹ flood.Methods : Water samples were collected from ۵ cities of Khuzestan province (Ahvaz, Ramhormoz, Mesjed Soleyman, Izeh and Baghmalek), ۷۰ samples were taken before the spring flood of ۲۰۱۹ and ۱۰ samples after it. Then the samples were to the cultured in Alkaline Peptone Water broth and TCBS agar. The exact species identification was done by performing Biochemical tests. The V.cholerae strains isolated were serotype by specific antisera to V.cholerae O۱.Results : In this study, all samples taken before the flood were non-O۱ V. cholerae, In these ۷۰ samples, Vibrio cholerae o۱ , Vibrio parahaemolyticus and Vibrio mimicus were not identified. In samples taken after floods in Khuzestan province, Nine samples were contaminated with non-O۱ Vibrio cholerae and one sample from Ahvaz (Karoon river) was contaminated with Vibrio Cholerae O۱. The identified strain was Ogawa.Conclusion : This study showed that non-O۱ V. choleara is the predominant species in surface waters of Khuzestan province. The results also show that Vibrio Cholerae O۱ exist in the surface water in this province

کلیدواژه ها:

Vibrio ، Flood ، Biochemical ، Serological ، khuzestan