Evaluation of the synergistic effects of zinc oxid nanocomposite film and doxycycline nanocomposite film against Staphylococcus aureus and Escherichia coli

Background and Aim : Infectious diseases are a global threat to human health. Excessive and inappropriate use of antibiotics has led to drug-resistant microbes that can neglect clinical treatment. Efforts are being made to develop safe and alternative antimicrobials to overcome such resistant microorganisms, and the birth of nanotechnology is promising to combat these organisms.Methods : In this study, according to the laboratory results obtained through disk diffusion method, determination of MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration), Fractional Inhibitory Concentration (FIC) for Confirmation of the synergistic effect of zinc oxide nanocomposites and doxycycline nanocomposites against Staphylococcus aureus, ۲۹۲۱۳ ATCC and Escherichia coli, ۳۵۲۱۸ ATCC was determined.Results : Minimum fractional inhibitory concentration to confirm the synergistic effect of zinc oxide nanocomposites and doxycycline nanocomposites against Staphylococcus aureus ۰.۰۳ + ۰.۰۷۵ mg /ml and FICI (Fractional Inhibitory Concentration Index) respectively ۰.۳۷۵ ,was observed for Escherichia coli with concentrations of ۰.۲۴ + ۰.۲۴ mg /ml and FICI ۰.۵, respectively.Conclusion : During the study and confirmation of the synergistic effect between zinc oxide nanocomposite film and doxycycline nanocomposite film, it can be said that these films had a synergistic effect against the studied bacteria and the greater effect of antibacterial agents on gram-positive bacteria than gram-negative bacteria is clearly known.

کلیدواژه ها:

Resistant microorganisms ، Nanocomposite films ، FIC ، Synergistic effect