Inhibitory Effects of Various Concentrations of Tetracycline and Trimethoprim on the Prevention of Congenital listeriosis in Balb/C mice

Background and Aim : Listeriosis is among the diseases occurring to humans, wild and domestic animals. Listeria monocytogenes, the etiologic agent of this disease, is one the most important food-borne pathogens. Given the limited information regarding the effects of various concentrations of different antibiotics on Listeria monocytogenes for in vivo studies, this study is designed. The aim of this study was to evaluate the inhibitory effects of different concentrations of tetracycline and trimethoprim on the prevention of congenital listeriosis in Balb/C mice Methods : In this experimental-analytical study, the effect of various concentrations of tetracycline and trimethoprim on congenital listeriosis was evaluated in ۴ groups of modelBalb/C mice, (treated with tetracycline, treated with trimethoprim, positive control and negative control). Minimum inhibitory concentration for Listeria monocytogenes was determinedin Muller-Hinton broth medium.For in vivo study, different dosages of tetracycline and trimethoprim were administered orally. Clinical evidence, sampling and culture methods were used for listeriosis identification Results : The obtained results showed that the percentage of live birth was higher in the treated mice than in the control groups. Using tetracycline and trimethoprim prevented abortion in treated Balb/C mice. For Trimethoprim and Tetracycline were effective for ۱۰۰% and ۷۴% prevention in abortion, respectively, as observed.Conclusion : Based on the results obtained in this study, it can be concluded thattrimethoprim antibiotic had more inhibitory effects than tetracycline on the abortion prevention in Balb/C mice.