Antimicrobial activity of plant essential oils Nano emulsions against Campylobacter jejuni

Background and Aim : This study aimed to examine the antimicrobial potential of three nanoemulsions (NE) of essential oils: Mentha piperita (MPNE), Zingiber officinale (ZONE), and Satureja khuzestanica (SKNE) against Campylobacter jejuni.Methods : Oil-in-water nanoemulsions were prepared using Tween ۸۰ and SDS as surfactant and cosurfactant respectively. The droplet size and size distribution (polydispersity index-PDI) of the nanoemulsion was measured using dynamic light scattering (VASCO۲ nanoparticle size analyzer/DLS). The Innate stability of nanoemulsion (the ability of a nanoemulsion to resist changes whiteout phase separation or creaming or flocculation) was assessed. Antibacterial effects of nanoemulsions were determined using Agar well diffusion test and Agar dilution method according to CLSI methods Results : Stable nanoemulsions containing small droplets (d <۶۰ nm) were formed. The MPNE, ZONE, and SKNE inhibition zones were ۱۶ ± ۰.۰, ۱۷.۵ ±۰.۵, ۳۰ ± ۱ mm, respectively. The minimum inhibitory concentration (MIC) results showed that the SKNE (MIC (۶.۲۵ µl/ml)) has more antibacterial properties against Campylobacter jejuni than MPNE and ZONE according to the MIC results (MIC (?۱۰۰µl/ml))Conclusion : The nanoemulsions of these essential oils displayed remarkable activity against C. jejuni, therefore, they could be used as natural anti-Campylobacter in the food industry.

کلیدواژه ها:

Nanoemulsions ، plant essential oils ، Campylobacter jejuni.